Host / isotype: Rabbit / IgG
Kind: Isotype control
Excitation / emission profile: View spectra
Concentration: 0.5mg / ml
Purification: Affinity chromatography
Storage buffer: PBS, pH 7.2, with 0.1% gelatin
Contains: 0.09% sodium azide
Storage conditions: 4 ° C, store in the dark, DO NOT FREEZE!
Description: Rabbit IgG polyclonal antibody is useful as an isotype control immunoglobulin.
Informed Applications: Rabbit IgG isotype control has been reported to be used as a negative control for immunofluorescent staining and flow cytometric analysis.
Proven Applications: This isotype control antibody has been tested by flow cytometric analysis. Use it at the same concentration as the experimental antibody.
Excitation: 488 nm; Emission: 520 nm; Laser: blue laser.
Filtration: 0.2 µm post-fabrication filtering.
The isotype of a primary antibody and the application in which it is being used can cause background staining. Background noise from the primary antibody may be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cellular autofluorescence. Isotype control antibodies can act as negative controls to help differentiate the non-specific background signal from the specific antibody signal because they do not have relevant specificity for a target antigen.
While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel changes. Isotype controls must match the primary antibody species and isotype so that the level of specific staining of the primary antibody can be accurately determined. If you are using directly labeled primary antibodies, the isotype control works best if it is conjugated to the same marker as the test antibody.