Oxidative damage of lipids caused by reactive oxygen species (ROS) plays an important role in some diseases, lesion of cell functions, and aging. Aldehydes such as malondialdehyde (MDA) and 4-hydroxy-2-nominal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxy octadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residue.
HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation. Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.
JaICA has developed the HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Hyogo University). This ELISA kit can be applied to human and animal urine, serum, and cultured cells.
Assay principle: Competitive ELISA (detection: 450 nm)
Specificity: Specific to N-epsilon-Hexanoyl-Lysine adduct
Measuring range: 2 – 700 nmol/L
Time to answer: Overnight and 2 hours.
Format: 96 wells (54 samples in single assay)
Applications: Urine, serum, and cultured cells from humans and animals.
Storage: Store at 2 – 8°C (don’t freeze).
Expiry: 2 years after the day of manufacturing.
Required but not provided: 50 µL micro pipettor and pipette tips
8-channel (50-200 µL) micropipettor and tips
8 or 12-synchronous multichannel pipet and reagent tray for multichannel pipet.
4-7 °C incubator
Microtiter plate reader (measuring wavelength 450 nm)