Biological source: human
Recombinant: expressed in HEK 293 cells
Test: ≥95% (SDS-PAGE)
Form: lyophilized powder
Power: 0.22 μg / ml
- Calculated molecular weight 25.7 kDa
- 28 kDa observed molecular weight (DTT reduced. Protein migrates due to glycosylation. Phe17 is the predicted N-terminal).
- Observed molecular weight 32 kDa
Packaging: 10 μg pack
Impurities: <1 EU / μg endotoxin (LAL test)
Conjugate: 6-His tagging (C-terminal)
UniProt access no: P09429
High mobility group protein B1 (HMGB1), also known as high mobility group 1 (HMG-1) protein and amphotericin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2, and HMGB3. HMGB1 is a histone-free architectural chromosomal protein that is ubiquitously present in all vertebrate nuclei and binds to double-stranded DNA without sequence specificity.
However, it can move between the nucleus and the cytoplasm. The protein is secreted by activated monocytes and macrophages. HMGB1 belongs to the superfamily of proteins of the high mobility group. The gene is mapped to human chromosome 13q12.
HMGB1 (high mobility group protein B1) has been used to identify factors that mask HMGB1 in human serum and plasma. It has been used to study HMGB1-mediated growth and motility in papillary thyroid cancer cells. Human HMGB1 / HMG1 has been used as a positive control to analyze the expression of the purified HMGB1 gene.
Biochemical / Physiological Actions
In the nucleus, HMGB1 (high mobility group protein B1) acts as a nucleosome stabilizer and a transcription regulator. It also exhibits chaperone activity and suppresses polyglutamine aggregation. It is also involved in inflammation and autophagy. HMGB1 can interact with toll-like receptor (TLR) 2 and 4, interleukin-1β, chemokine ligand 12 (C-X-C motif) (CXCL12), and pathogen-associated molecular pattern molecules (PAMP). It also functions as a damage-associated molecular pattern molecule (DAMP).
The mechanism of inflammation and damage binds to TLR4, which mediates the activation of HMGB1-dependent macrophage cytokine release. This places HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy. In the presence of Epstein-Barr virus (EBV) infection, HMGB1 is up-regulated and is responsible for the proliferation of human nasopharyngeal carcinoma cells. It is involved in the migration and invasion of cancer cells.
Lyophilized from a 0.22 µm filtered solution in PBS. Generally, 5-8% mannitol or trehalose is added as a protector before lyophilization.
Centrifuge the vial before opening. Reconstitute in sterile PBS, pH 7.4 at a concentration of 50 μg / mL. Do not shake. This solution can be stored at 2-8 ° C for up to 1 month. For long-term storage, it is recommended to store at -20 ° C.