Click on Chemistry for Imaging in-situ Protein Palmitoylation through the Asexual Levels of Plasmodium falciparum
Palmitoylation refers back to the modification of the cysteine thiols in proteins by fatty acids, mostly palmitic acid, by ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has lately emerged as an important post-translational modification in malarial parasites. The expression and exercise of palmitoyl transferases fluctuate throughout totally different developmental phases of the malarial parasite’s life cycle. The abundance of palmitoylated proteins at a given stage is a measure of total PAT exercise. The PAT exercise also can change in response to exterior indicators or inhibitors.
Right here, we describe a protocol to ‘picture’ palmitoyl-transferase exercise through the asexual phases utilizing Click on Chemistry and fluorescence microscopy. This methodology relies on metabolic labeling of a clickable analog of palmitic acid by parasitic cells, adopted by CuAAC (Copper-catalyzed Alkyne-Azide Cycloaddition response) Click on Chemistry to render palmitoylated proteins fluorescent. Fluorescence permits the quantitation of intracellular palmitoylation in parasite cells throughout numerous improvement phases. Utilizing this methodology, we noticed that intracellular palmitoylation will increase because the parasite transitions from ring to schizont phases and seems to be most plentiful through the schizont phases in Plasmodium falciparum.
immune subtraction for improved decision in serum protein immunofixation electrophoresis and antibody isotype willpower in a affected person with autoantibody
Heavy chain isotypes of low stage monoclonal immunoglobulins are typically obscured in serum immunofixation electrophoresis (SIFE) by a heavy background of polyclonal immunoglobulins. Nevertheless, correct willpower of the heavy chain isotype is important for an entire analysis, as isotype willpower of autoantibodies could have relevance in figuring out therapeutic procedures. Immune subtraction (IS) was employed in a affected person with neuropathy and GD1a autoantibody. IS allowed identification of the cognate heavy chain associated to a lambda mild chain restriction famous on preliminary SIFE in addition to isotype willpower of the autoantibody.
Antisera particular to particular person heavy and lightweight chains have been used for depletion of particular immunoglobulin varieties. Depletion of kappa mild chain related immunoglobulins allowed unequivocal willpower of the isotype of lambda mild chain-associated low stage monoclonal band to be IgG Lambda. Selective depletion of kappa, lambda, gamma and mu heavy chain immunoglobulins was employed to find out IgG Kappa isotype of the auto-antibody.

Sp100 Nuclear Antigen (Sp100) Polyclonal Antibody (Mouse), FITC |
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4-PAA326Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Sp100 Nuclear Antigen (Sp100). This antibody is labeled with FITC. |
Sp100 Nuclear Antigen (Sp100) Polyclonal Antibody (Rat), FITC |
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4-PAA326Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Sp100 Nuclear Antigen (Sp100). This antibody is labeled with FITC. |
Ribonuclease III, Nuclear (RNASEN) Polyclonal Antibody (Human), FITC |
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4-PAD163Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Ribonuclease III, Nuclear (RNASEN). This antibody is labeled with FITC. |
Human Nuclear pore complex protein Nup153 (NUP153) |
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1-CSB-EP016190HU | Cusabio |
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Description: Recombinant Human Nuclear pore complex protein Nup153(NUP153),partial expressed in E.coli |
Recombinant human Nuclear pore complex protein Nup133 |
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P2220 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for human Nuclear pore complex protein Nup133 |
Nuclear pore complex protein Nup98 (315-360) |
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HY-P1730 | MedChemExpress | 1mg | EUR 1368 |
Nuclear pore membrane glycoprotein 210 Antibody (FITC) |
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20-abx107147 | Abbexa |
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Proliferating Cell Nuclear Antigen (PCNA) Polyclonal Antibody (Human), FITC |
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4-CAA591Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Proliferating Cell Nuclear Antigen (PCNA). This antibody is labeled with FITC. |
Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody (Human), FITC |
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4-CAA591Hu22-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Proliferating Cell Nuclear Antigen (PCNA). This antibody is labeled with FITC. |
Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Human), FITC |
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4-PAC791Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with FITC. |
Thymocyte Nuclear Protein 1 (THYN1) Polyclonal Antibody (Rat), FITC |
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4-PAC791Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Thymocyte Nuclear Protein 1 (THYN1). This antibody is labeled with FITC. |
Nuclear Factor Kappa B (NFkB) Polyclonal Antibody (Chicken), FITC |
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4-PAB824Ga01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Chicken Nuclear Factor Kappa B (NFkB). This antibody is labeled with FITC. |
Nuclear Factor Kappa B (NFkB) Polyclonal Antibody (Human), FITC |
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4-PAB824Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Factor Kappa B (NFkB). This antibody is labeled with FITC. |
Nuclear Factor Kappa B (NFkB) Polyclonal Antibody (Human), FITC |
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4-PAB824Hu02-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Factor Kappa B (NFkB). This antibody is labeled with FITC. |
Nuclear Factor Kappa B (NFkB) Polyclonal Antibody (Rat), FITC |
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4-PAB824Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Factor Kappa B (NFkB). This antibody is labeled with FITC. |
Nuclear Factor Kappa B2 (NFkB2) Polyclonal Antibody (Chicken), FITC |
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4-PAB825Ga01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Chicken Nuclear Factor Kappa B2 (NFkB2). This antibody is labeled with FITC. |
Nuclear Factor Kappa B2 (NFkB2) Polyclonal Antibody (Mouse), FITC |
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4-PAB825Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Nuclear Factor Kappa B2 (NFkB2). This antibody is labeled with FITC. |
Nuclear Factor Kappa B2 (NFkB2) Polyclonal Antibody (Mouse), FITC |
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4-PAB825Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Nuclear Factor Kappa B2 (NFkB2). This antibody is labeled with FITC. |
Nuclear Factor I/B (NFIB) Polyclonal Antibody (Human), FITC |
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4-PAF663Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Factor I/B (NFIB). This antibody is labeled with FITC. |
Nuclear Factor I/X (NFIX) Polyclonal Antibody (Rat), FITC |
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4-PAF665Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Factor I/X (NFIX). This antibody is labeled with FITC. |
Nuclear Receptor Coactivator 3 (NCOA3) Polyclonal Antibody (Human), FITC |
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4-PAE798Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Receptor Coactivator 3 (NCOA3). This antibody is labeled with FITC. |
Nuclear Receptor Coactivator 3 (NCOA3) Polyclonal Antibody (Rat), FITC |
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4-PAE798Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Receptor Coactivator 3 (NCOA3). This antibody is labeled with FITC. |
Germ Cell Nuclear Factor (GCNF) Polyclonal Antibody (Human), FITC |
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4-PAC509Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Germ Cell Nuclear Factor (GCNF). This antibody is labeled with FITC. |
Nuclear Factor I/A (NFIA) Polyclonal Antibody (Human), FITC |
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4-PAC655Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Factor I/A (NFIA). This antibody is labeled with FITC. |
Nuclear Transport Factor 2 (NUTF2) Polyclonal Antibody (Human), FITC |
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4-PAC671Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Transport Factor 2 (NUTF2). This antibody is labeled with FITC. |
Proliferating Cell Nuclear Antigen (PCNA) Polyclonal Antibody (Human), FITC |
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4-PAA591Mi01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Proliferating Cell Nuclear Antigen (PCNA). This antibody is labeled with FITC. |
Nuclear Matrix Protein 4 (NMP4) Polyclonal Antibody (Human), FITC |
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4-PAB095Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Matrix Protein 4 (NMP4). This antibody is labeled with FITC. |
Mediator Complex Subunit 1 (MED1) Polyclonal Antibody (Human), FITC |
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4-PAC238Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Mediator Complex Subunit 1 (MED1). This antibody is labeled with FITC. |
Nuclear Pore Complex-Interacting Protein-Like 2 (NPIPL2) Antibody |
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abx034938-400ul | Abbexa | 400 ul | EUR 627.6 |
Nuclear Pore Complex-Interacting Protein-Like 2 (NPIPL2) Antibody |
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abx034938-80l | Abbexa | 80 µl | EUR 343.2 |
Nuclear Mitotic Apparatus Protein 1 (NUMA1) Monoclonal Antibody (Human), FITC |
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4-MAC332Hu22-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Nuclear Mitotic Apparatus Protein 1 (NUMA1). This antibody is labeled with FITC. |
Hepatocyte Nuclear Factor 4 Alpha (HNF4a) Polyclonal Antibody (Human), FITC |
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4-PAA418Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Hepatocyte Nuclear Factor 4 Alpha (HNF4a). This antibody is labeled with FITC. |
Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) Polyclonal Antibody (Human), FITC |
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4-PAA323Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1). This antibody is labeled with FITC. |
Small Nuclear Ribonucleoprotein Polypeptide C (SNRPC) Polyclonal Antibody (Human), FITC |
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4-PAB374Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Small Nuclear Ribonucleoprotein Polypeptide C (SNRPC). This antibody is labeled with FITC. |
Photoreceptor Cell Specific Nuclear Receptor (PNR) Polyclonal Antibody (Human), FITC |
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4-PAD956Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Photoreceptor Cell Specific Nuclear Receptor (PNR). This antibody is labeled with FITC. |
Nuclear Receptor Related Protein 1 (NURR1) Polyclonal Antibody (Human), FITC |
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4-PAD964Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Receptor Related Protein 1 (NURR1). This antibody is labeled with FITC. |
Hepatocyte Nuclear Factor 1 Beta (HNF1b) Polyclonal Antibody (Human), FITC |
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4-PAG776Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Hepatocyte Nuclear Factor 1 Beta (HNF1b). This antibody is labeled with FITC. |
Nuclear RNA Export Factor 1 (NXF1) Polyclonal Antibody (Rat), FITC |
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4-PAC208Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear RNA Export Factor 1 (NXF1). This antibody is labeled with FITC. |
Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1) Polyclonal Antibody (Human, Pig), FITC |
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4-PAC252Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Pig Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1). This antibody is labeled with FITC. |
Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1) Polyclonal Antibody (Human, Mouse), FITC |
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4-PAC252Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1). This antibody is labeled with FITC. |
Myeloid Cell Nuclear Differentiation Antigen (MNDA) Polyclonal Antibody (Human), FITC |
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4-PAB775Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Myeloid Cell Nuclear Differentiation Antigen (MNDA). This antibody is labeled with FITC. |
Nuclear Factor Kappa B (NFkB) Polyclonal Antibody (Human, Mouse), FITC |
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4-PAB824Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse Nuclear Factor Kappa B (NFkB). This antibody is labeled with FITC. |
Nuclear Transcription Factor Y Gamma (NFYC) Polyclonal Antibody (Human), FITC |
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4-PAF667Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Transcription Factor Y Gamma (NFYC). This antibody is labeled with FITC. |
Nuclear Distribution Gene C Homolog (NUDC) Polyclonal Antibody (Human), FITC |
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4-PAJ712Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Distribution Gene C Homolog (NUDC). This antibody is labeled with FITC. |
Nuclear Mitotic Apparatus Protein 1 (NUMA1) Polyclonal Antibody (Human), FITC |
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4-PAC332Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Mitotic Apparatus Protein 1 (NUMA1). This antibody is labeled with FITC. |
Sp140 Nuclear Body Protein (SP140) Polyclonal Antibody (Human, Rat), FITC |
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4-PAN896Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Sp140 Nuclear Body Protein (SP140). This antibody is labeled with FITC. |
Trafficking Protein Particle Complex 2 (TRAPPC2) Polyclonal Antibody (Human), FITC |
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4-PAC808Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Trafficking Protein Particle Complex 2 (TRAPPC2). This antibody is labeled with FITC. |
Pyruvate Dehydrogenase Complex Component X (PDHX) Polyclonal Antibody (Mouse), FITC |
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4-PAH426Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pyruvate Dehydrogenase Complex Component X (PDHX). This antibody is labeled with FITC. |
Succinate Dehydrogenase Complex Subunit B (SDHB) Polyclonal Antibody (Mouse), FITC |
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4-PAJ756Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Succinate Dehydrogenase Complex Subunit B (SDHB). This antibody is labeled with FITC. |
Succinate Dehydrogenase Complex Subunit A (SDHA) Polyclonal Antibody (Mouse), FITC |
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4-PAJ784Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Succinate Dehydrogenase Complex Subunit A (SDHA). This antibody is labeled with FITC. |
Succinate Dehydrogenase Complex Subunit C (SDHC) Polyclonal Antibody (Mouse), FITC |
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4-PAK213Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Succinate Dehydrogenase Complex Subunit C (SDHC). This antibody is labeled with FITC. |
Succinate Dehydrogenase Complex Subunit D (SDHD) Polyclonal Antibody (Mouse), FITC |
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4-PAK214Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Succinate Dehydrogenase Complex Subunit D (SDHD). This antibody is labeled with FITC. |
Aryl Hydrocarbon Receptor Nuclear Translocator 2 (ARNT2) Polyclonal Antibody (Human), FITC |
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4-PAC301Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Aryl Hydrocarbon Receptor Nuclear Translocator 2 (ARNT2). This antibody is labeled with FITC. |
Nuclear Autoantigenic Sperm Protein, Histone Binding (NASP) Polyclonal Antibody (Rat), FITC |
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4-PAC652Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Autoantigenic Sperm Protein, Histone Binding (NASP). This antibody is labeled with FITC. |
HAUS Augmin Like Complex Subunit 7 (HAUS7) Polyclonal Antibody (Human), FITC |
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4-PAM623Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human HAUS Augmin Like Complex Subunit 7 (HAUS7). This antibody is labeled with FITC. |
HAUS Augmin Like Complex Subunit 7 (HAUS7) Polyclonal Antibody (Mouse), FITC |
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4-PAM623Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse HAUS Augmin Like Complex Subunit 7 (HAUS7). This antibody is labeled with FITC. |
HAUS Augmin Like Complex Subunit 7 (HAUS7) Polyclonal Antibody (Rat), FITC |
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4-PAM623Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat HAUS Augmin Like Complex Subunit 7 (HAUS7). This antibody is labeled with FITC. |
Pyruvate Dehydrogenase Complex Component X (PDHX) Polyclonal Antibody (Human, Pig), FITC |
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4-PAH426Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Pig Pyruvate Dehydrogenase Complex Component X (PDHX). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class I E (MHCE) Polyclonal Antibody (Human), FITC |
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4-PAB752Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Major Histocompatibility Complex Class I E (MHCE). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class I G (MHCG) Polyclonal Antibody (Human), FITC |
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4-PAB856Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Major Histocompatibility Complex Class I G (MHCG). This antibody is labeled with FITC. |
Achaete Scute Complex Like Protein 1 (ASCL1) Polyclonal Antibody (Human), FITC |
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4-PAJ867Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Achaete Scute Complex Like Protein 1 (ASCL1). This antibody is labeled with FITC. |
BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3) Polyclonal Antibody (Human), FITC |
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4-PAN348Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human BRCA1/BRCA2 Containing Complex Subunit 3 (BRCC3). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B (RANk) Monoclonal Antibody (Human), FITC |
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4-MAC057Hu22-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Receptor Activator Of Nuclear Factor Kappa B (RANk). This antibody is labeled with FITC. |
Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) Polyclonal Antibody (Rat), FITC |
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4-PAD468Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL). This antibody is labeled with FITC. |
Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Polyclonal Antibody (Human, Rat, Pig), FITC |
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4-PAD470Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat, Pig Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). This antibody is labeled with FITC. |
Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A) Polyclonal Antibody (Mouse), FITC |
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4-PAJ636Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A). This antibody is labeled with FITC. |
Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1) Polyclonal Antibody (Human, Rat, Pig), FITC |
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4-PAB128Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat, Pig Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B (RANk) Polyclonal Antibody (Mouse), FITC |
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4-PAC057Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Receptor Activator Of Nuclear Factor Kappa B (RANk). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II Invariant Chain (MHCDG) Polyclonal Antibody (Human), FITC |
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4-PAB369Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Major Histocompatibility Complex Class II Invariant Chain (MHCDG). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II Invariant Chain (MHCDG) Polyclonal Antibody (Rat), FITC |
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4-PAB369Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Major Histocompatibility Complex Class II Invariant Chain (MHCDG). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Bovine), FITC |
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4-PAC166Bo01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Dog), FITC |
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4-PAC166Ca01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Dog Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Pig), FITC |
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4-PAC166Po01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Rabbit), FITC |
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4-PAC166Rb51-FITC | Cloud-Clone |
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Description: A Guinea polyclonal antibody against Rabbit Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Mouse), FITC |
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4-PAC166Si01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with FITC. |
Adaptor Related Protein Complex 2 Mu 1 (AP2m1) Polyclonal Antibody (Human), FITC |
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4-PAG107Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Adaptor Related Protein Complex 2 Mu 1 (AP2m1). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class I C (MHCC) Polyclonal Antibody (Human, Pig), FITC |
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4-PAC086Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Pig Major Histocompatibility Complex Class I C (MHCC). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class I B (MHCB) Polyclonal Antibody (Human, Pig), FITC |
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4-PAC092Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Pig Major Histocompatibility Complex Class I B (MHCB). This antibody is labeled with FITC. |
Mouse Nuclear pore complex protein Nup155, Nup155 ELISA KIT |
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ELI-44221m | Lifescience Market | 96 Tests | EUR 1038 |
Human Nuclear pore complex protein Nup214, NUP214 ELISA KIT |
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ELI-44222h | Lifescience Market | 96 Tests | EUR 988.8 |
Bovine Nuclear pore complex protein Nup93, NUP93 ELISA KIT |
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ELI-44229b | Lifescience Market | 96 Tests | EUR 1113.6 |
Mouse Nuclear pore complex protein Nup133, Nup133 ELISA KIT |
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ELI-44499m | Lifescience Market | 96 Tests | EUR 1038 |
Mouse Nuclear pore complex protein Nup93, Nup93 ELISA KIT |
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ELI-44561m | Lifescience Market | 96 Tests | EUR 1038 |
Human Nuclear pore complex protein Nup153, NUP153 ELISA KIT |
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ELI-44867h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Nuclear pore complex protein Nup133, NUP133 ELISA KIT |
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ELI-46087h | Lifescience Market | 96 Tests | EUR 988.8 |
Mouse Nuclear pore complex protein Nup107, Nup107 ELISA KIT |
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ELI-38239m | Lifescience Market | 96 Tests | EUR 1038 |
Human Nuclear pore complex protein Nup160, NUP160 ELISA KIT |
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ELI-38240h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Nuclear pore complex protein Nup205, NUP205 ELISA KIT |
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ELI-38242h | Lifescience Market | 96 Tests | EUR 988.8 |
Mouse Nuclear pore complex protein Nup88, Nup88 ELISA KIT |
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ELI-35536m | Lifescience Market | 96 Tests | EUR 1038 |
Human Nuclear pore complex protein Nup85, NUP85 ELISA KIT |
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ELI-36779h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Nuclear pore complex protein Nup88, NUP88 ELISA KIT |
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ELI-36780h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Nuclear pore complex protein Nup93, NUP93 ELISA KIT |
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ELI-36781h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Nuclear pore complex protein Nup107, NUP107 ELISA KIT |
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ELI-36840h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Nuclear pore complex protein Nup50, NUP50 ELISA KIT |
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ELI-22432h | Lifescience Market | 96 Tests | EUR 988.8 |
Bovine Nuclear pore complex protein Nup85, NUP85 ELISA KIT |
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ELI-23491b | Lifescience Market | 96 Tests | EUR 1113.6 |
Mouse Nuclear pore complex protein Nup85, Nup85 ELISA KIT |
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ELI-23492m | Lifescience Market | 96 Tests | EUR 1038 |
Human Nuclear pore complex protein Nup155, NUP155 ELISA KIT |
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ELI-15040h | Lifescience Market | 96 Tests | EUR 988.8 |
Mouse Nuclear pore complex protein Nup50, Nup50 ELISA KIT |
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ELI-15055m | Lifescience Market | 96 Tests | EUR 1038 |
Mouse Nuclear pore complex protein Nup54, Nup54 ELISA KIT |
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ELI-21319m | Lifescience Market | 96 Tests | EUR 1038 |
Mouse Nuclear pore complex protein Nup160, Nup160 ELISA KIT |
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ELI-21357m | Lifescience Market | 96 Tests | EUR 1038 |
Mouse Nuclear pore complex protein Nup214, Nup214 ELISA KIT |
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ELI-21358m | Lifescience Market | 96 Tests | EUR 1038 |
Human Nuclear pore complex- interacting protein, NPIP ELISA KIT |
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ELI-16689h | Lifescience Market | 96 Tests | EUR 988.8 |
Nuclear pore membrane glycoprotein 210 Polyclonal Conjugated Antibody |
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C42276 | SAB | 100ul | EUR 476.4 |
Receptor Activator Of Nuclear Factor Kappa B (RANk) Monoclonal Antibody (Human, Rat), FITC |
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4-MAC057Hu21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human, Rat Receptor Activator Of Nuclear Factor Kappa B (RANk). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Monoclonal Antibody (Human), FITC |
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4-MAA855Hu21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Monoclonal Antibody (Rat), FITC |
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4-MAA855Ra26-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody (Mouse), FITC |
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4-PAA855Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody (Rat), FITC |
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4-PAA855Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with FITC. |
Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) Polyclonal Antibody (Rat), FITC |
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4-PAD946Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2). This antibody is labeled with FITC. |
Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1) Polyclonal Antibody (Rat), FITC |
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4-PAD966Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1). This antibody is labeled with FITC. |
Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) Polyclonal Antibody (Human), FITC |
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4-PAL942Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). This antibody is labeled with FITC. |
Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) Polyclonal Antibody (Human), FITC |
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4-PAL947Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). This antibody is labeled with FITC. |
Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) Polyclonal Antibody (Rat), FITC |
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4-PAL947Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). This antibody is labeled with FITC. |
Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A) Polyclonal Antibody (Human, Rat), FITC |
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4-PAJ636Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A). This antibody is labeled with FITC. |
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody (Human), FITC |
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4-PAB608Hu02-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). This antibody is labeled with FITC. |
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody (Rat), FITC |
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4-PAB608Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). This antibody is labeled with FITC. |
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody (Rat), FITC |
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4-PAB608Ra02-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B (RANk) Polyclonal Antibody (Human, Mouse), FITC |
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4-PAC057Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse Receptor Activator Of Nuclear Factor Kappa B (RANk). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B (RANk) Polyclonal Antibody (Human, Rat), FITC |
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4-PAC057Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Receptor Activator Of Nuclear Factor Kappa B (RANk). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Monoclonal Antibody (Rhesus monkey), FITC |
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4-MAC166Si21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Rhesus monkey Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II Invariant Chain (MHCDG) Polyclonal Antibody (Mouse, Rat), FITC |
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4-PAB369Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse, Rat Major Histocompatibility Complex Class II Invariant Chain (MHCDG). This antibody is labeled with FITC. |
Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) Polyclonal Antibody (Mouse), FITC |
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4-PAL761Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). This antibody is labeled with FITC. |
ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b) Polyclonal Antibody (Rat), FITC |
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4-PAG387Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Human, Mouse), FITC |
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4-PAC166Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) Polyclonal Antibody (Human), FITC |
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4-PAC167Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). This antibody is labeled with FITC. |
Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) Polyclonal Antibody (Mouse), FITC |
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4-PAC167Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). This antibody is labeled with FITC. |
Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) Polyclonal Antibody (Mouse), FITC |
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4-PAJ599Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Actin Related Protein 2/3 Complex Subunit 4 (ARPC4). This antibody is labeled with FITC. |
Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) Polyclonal Antibody (Rat), FITC |
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4-PAJ599Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Actin Related Protein 2/3 Complex Subunit 4 (ARPC4). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Monoclonal Antibody (Human, Rat), FITC |
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4-MAA855Ra21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human, Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Monoclonal Antibody (Human, Rat), FITC |
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4-MAA855Ra24-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human, Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with FITC. |
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody (Human, Mouse), FITC |
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4-PAA855Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with FITC. |
Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP) Polyclonal Antibody (Human), FITC |
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4-PAB953Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP). This antibody is labeled with FITC. |
Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP) Polyclonal Antibody (Mouse), FITC |
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4-PAB953Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP). This antibody is labeled with FITC. |
Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP) Polyclonal Antibody (Mouse), FITC |
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4-PAB953Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP). This antibody is labeled with FITC. |
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody (Human, Pig), FITC |
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4-PAB608Hu03-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Pig Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). This antibody is labeled with FITC. |
Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) Polyclonal Antibody (Human, Mouse), FITC |
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4-PAJ599Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse Actin Related Protein 2/3 Complex Subunit 4 (ARPC4). This antibody is labeled with FITC. |
Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) Polyclonal Antibody (Human, Rat), FITC |
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4-PAJ601Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Rat Actin Related Protein 2/3 Complex Subunit 2 (ARPC2). This antibody is labeled with FITC. |
SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody |
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20-abx123660 | Abbexa |
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SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody |
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20-abx126518 | Abbexa |
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SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody |
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abx025879-400ul | Abbexa | 400 ul | EUR 627.6 |
SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody |
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abx025879-80l | Abbexa | 80 µl | EUR 343.2 |
SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody |
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abx237677-100ug | Abbexa | 100 ug | EUR 610.8 |
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody (Human, Mouse, Rat), FITC |
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4-PAB608Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). This antibody is labeled with FITC. |
Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) Polyclonal Antibody (Human, Mouse, Rat), FITC |
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4-PAL761Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). This antibody is labeled with FITC. |
ELISA kit for Rat Nuclear pore complex protein Nup153 (NUP153) |
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KTE100879-48T | Abbkine | 48T | EUR 398.4 |
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Rat Nuclear pore complex protein Nup153 (NUP153) |
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KTE100879-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Rat Nuclear pore complex protein Nup153 (NUP153) |
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KTE100879-96T | Abbkine | 96T | EUR 646.8 |
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Human Nuclear pore complex protein Nup98- Nup96, NUP98 ELISA KIT |
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ELI-13775h | Lifescience Market | 96 Tests | EUR 988.8 |
Nuclear pore membrane glycoprotein 210 Antibody |
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20-abx110055 | Abbexa |
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Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-100ug |
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QP6439-ec-100ug | EnQuireBio | 100ug | EUR 489.6 |
Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-10ug |
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QP6439-ec-10ug | EnQuireBio | 10ug | EUR 240 |
Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-1mg |
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QP6439-ec-1mg | EnQuireBio | 1mg | EUR 1958.4 |
Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-200ug |
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QP6439-ec-200ug | EnQuireBio | 200ug | EUR 760.8 |
Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-500ug |
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QP6439-ec-500ug | EnQuireBio | 500ug | EUR 1272 |
Recombinant Human Nuclear pore complex protein Nup153 Protein, His, E.coli-50ug |
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QP6439-ec-50ug | EnQuireBio | 50ug | EUR 315.6 |
Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), FITC |
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4-MAO843Ge21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with FITC. |
Goat Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-FITC conjugate |
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GFP11-FITC | Alpha Diagnostics | 100 ug | EUR 416.4 |
Monoclonal Anti-Green Fluorescent Proteins (GFP, A. victoria) protein IgG-FITC conjugate |
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GFP12-FITC | Alpha Diagnostics | 0.5 ml | EUR 489.6 |
Antifreeze Protein Supplementation In the course of the Warming of Vitrified Bovine Ovarian Tissue Can Enhance the Ovarian Tissue High quality After Xenotransplantation
The prevalence of ice crystallization throughout ovarian tissue (OT) cryopreservation causes unavoidable cryodamage, and ice recrystallization through the warming is extra detrimental than ice crystallization. Right here, we investigated that antifreeze protein (AFP) remedy through the warming process can enhance the bovine OT high quality after xenotransplantation (XT). Bovine OTs (n=120) have been evenly assigned to 4 teams: recent, vitrified-warmed, vitrified-warmed with 10 mg/mL Leucosporidium ice-binding protein (LeIBP, a kind of AFP) (LeIBP-10), and vitrified-warmed with 20 mg/mL LeIBP (LeiBP-20). LeIBPs have been added to the primary warming answer. Twenty items of OTs have been assigned to every class. The remaining 10 OTs from every class have been assigned to the XT-Recent management, XT-Vitrified-warmed management, XT-LeIBP-10, and XT-LeIBP-20 teams, respectively, and xenotransplanted to 9-week-old ovariectomized nude mice for one week.
LeIBP remedy through the warming step elevated morphological follicle normality and decreased apoptotic follicle ratios after vitrification-warming and XT. The XT-vitrified-warmed management group confirmed considerably decreased microvessel density and elevated fibrosis when in comparison with that of the XT-fresh group. Microvessel density and fibrosis have been recovered in each LeIBP handled teams. There was no important distinction between the LeIBP-10 and LeIBP-20 teams in all outcomes. AFP remedy through the warming process can stop OT harm, and enhance ovarian follicle morphology and apoptosis in each the vitrified-warmed bovine OT and its graft. After affirmation in a human research, AFPs can probably be utilized to human OT cryopreservation to cut back cryodamage and enhance the OT high quality.
Attenuated Induction of the Unfolded Protein Response in Grownup Human Main Astrocytes in Response to Recurrent Low Glucose
Goals/speculation: Recurrent hypoglycaemia (RH) is a serious side-effect of intensive insulin remedy for individuals with diabetes. Modifications in hypoglycaemia sensing by the mind contribute to the event of impaired counterregulatory responses to and consciousness of hypoglycaemia. Little is understood concerning the intrinsic adjustments in human astrocytes in response to acute and recurrent low glucose (RLG) publicity.
Strategies: Human main astrocytes (HPA) have been uncovered to zero, one, three or 4 bouts of low glucose (0.1 mmol/l) for 3 hours per day for 4 days to imitate RH. On the fourth day, DNA and RNA have been collected. Differential gene expression and ontology analyses have been carried out utilizing DESeq2 and GOseq, respectively. DNA methylation was assessed utilizing the Infinium MethylationEPIC BeadChip platform.
Outcomes: 24 differentially expressed genes (DEGs) have been detected (after correction for a number of comparisons). One bout of low glucose publicity had the most important impact on gene expression. Pathway analyses revealed that endoplasmic-reticulum (ER) stress-related genes corresponding to HSPA5, XBP1, and MANF, concerned within the unfolded protein response (UPR), have been all considerably elevated following low glucose (LG) publicity, which was diminished following RLG. There was little correlation between differentially methylated positions and adjustments in gene expression but the variety of bouts of LG publicity produced distinct methylation signatures.
Conclusions/interpretation: These knowledge counsel that publicity of human astrocytes to transient LG triggers activation of genes concerned within the UPR linked to endoplasmic reticulum (ER) stress. Following RLG, the activation of UPR associated genes was diminished, suggesting attenuated ER stress. This can be a consequence of a profitable metabolic adaptation, as beforehand reported, that higher preserves intracellular vitality ranges and a decreased necessity for the UPR.
Doxorubicin/Nucleophosmin Binding Protein-Conjugated Nanoparticle Enhances Anti-leukemia Exercise in Acute Lymphoblastic Leukemia Cells in vitro and in vivo
Acute lymphoblastic leukemia (ALL) is an aggressive malignancy. Adults with ALL have greater than 50% relapse charges. We’ve got beforehand validated that overexpression of nucleophosmin (NPM) is concerned within the multidrug resistance (MDR) improvement throughout ALL; and a synthetically engineered recombinant NPM binding protein (NPMBP) has been developed in our group; NPMBP and doxorubicin (DOX) might be conjugated in a nanoparticle-based drug supply system named DOX-PMs-NPMBP to counteract MDR throughout ALL. Right here, we evaluated the antileukemia potential of DOX-PMs-NPMBP in resistant ALL cells. This research demonstrates that DOX-PMs-NPMBP considerably enhances chemosensitivity to DOX in ALL cells.
Regardless of at variable concentrations, each resistant and first ALL cells from relapsed sufferers have been delicate to DOX-PMs-NPMBP. Intimately, the half maximal inhibitory focus (IC50) values of DOX-PMs-NPMBP have been between 1.6- and seven.0-fold decrease than these of DOX in cell traces and first ALL cells, respectively; and apoptotic cells ratio was over 2-fold increased in DOX-PMs-NPMBP than DOX. Mechanistically, p53-driven apoptosis induction and cell cycle arrest performed important function in DOX-PMs-NPMBP-induced anti-leukemia results. Furthermore, DOX-PMs-NPMBP considerably inhibited tumor development and extended mouse survival of ALL xenograft fashions; and no systemic toxicity prevalence was noticed after remedy throughout follow-up. In conclusion, these knowledge point out that DOX-PMs-NPMBP could considerably exert development inhibition and apoptosis induction, and markedly enhance DOX antileukemia exercise in resistant ALL cells. This novel drug supply system could also be useful to develop as a brand new therapeutic technique in opposition to multidrug resistant ALL.